Composite

Part:BBa_K2598009:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-20)


Blue light sensor YF1 and fixJ under constitutive promoter Lacclq35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 1259
    Illegal NgoMIV site found at 1552
    Illegal NgoMIV site found at 1646
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1316
    Illegal BsaI.rc site found at 200


Design Notes

No codon optimization has been done.


Source

YF1 is a fusion protein of YtvA (B subtilis) and FixL (B japonicum).FixJ comes from the genome of Bradyrhizobium japonicum

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Moglich, A., Ayers, R. A. & Moffat, K. Design and signaling mechanism of light-regulated histidine kinases. J Mol Biol 385, 1433-1444, (2009).