Composite
Part:BBa_K2598009:Design
Designed by: Matthew Gao Group: iGEM18_UCAS-China (2018-09-20)
Blue light sensor YF1 and fixJ under constitutive promoter Lacclq35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 587
Illegal NgoMIV site found at 659
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 1259
Illegal NgoMIV site found at 1552
Illegal NgoMIV site found at 1646
Illegal AgeI site found at 301
Illegal AgeI site found at 1427 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1316
Illegal BsaI.rc site found at 200
Design Notes
No codon optimization has been done.
Source
YF1 is a fusion protein of YtvA (B subtilis) and FixL (B japonicum).FixJ comes from the genome of Bradyrhizobium japonicum
References
Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)
Moglich, A., Ayers, R. A. & Moffat, K. Design and signaling mechanism of light-regulated histidine kinases. J Mol Biol 385, 1433-1444, (2009).